Micro-fabricated cell trap for visualizing non-adherent cells in a perfusion chamber Femtosecond pulsed laser micro-fabricated cell traps used capture non-adherent GFP expressing MD43 mouse T lymphocytes in a perfusion chamber mounted onto a microscope stage. Fluorescence over transmission images captured with an Olympus FV300 confocal. Imaged on an inverted microscope stage using a Bioptech FCS2 temperature controlled perfusion chamber. |
Direction of media perfusion |