The
goal of this project is to provide an integrated GM process to facilitate and
direct bioremediation in the presence of plants (rhizoremediation), by
·
developing and evaluating new GM rhizoremediation strains for in
situ bioremediation,
·
developing and evaluating molecular tools for quantifying GM
bacteria used in rhizoremediation under in situ conditions.
·
elucidating the fate of the pollutant (and key intermediates)
within the system "plant and soil"
·
extensively testing the new system in vitro, in microcosms and, in contained lysimeters and
in the field
·
preparing a detailed risk analysis of the GM
based bioprocess, and by
·
preparing a set of guideline for authorities and end user/site owners.
Milestones and expected results.
Integrated
biosensor strains will be constructed facilitating in
situ monitoring of PCB remediation. In particular, these tools will allow
efficacy monitoring of bioremediation inoculants. The findings will lead to a
better understanding of those measures to be applied for optimisation of
phytobioremediation technology.
Novel
molecular tools will be developed for the identification and unambiguous
quantification of genetically modified (GM) bacterial strains in the
environment. A detailed impact assessment on the use of the GM strains will
underpin the preparation of guidelines on the safe use of this GM technology.
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