Testing Integrated GM-Rhizoremediation systems for soil bioremediation (GM-Rhizoremediation)


The goal of this project is to provide an integrated GM process to facilitate and direct bioremediation in the presence of plants (rhizoremediation), by 

      developing and evaluating new GM rhizoremediation strains for in situ bioremediation,  

  developing and evaluating molecular tools for quantifying GM bacteria used in rhizoremediation under in situ conditions.

        elucidating the fate of the pollutant (and key intermediates) within the system "plant and soil"

        extensively testing the new system in vitro, in microcosms and, in contained lysimeters and in the field

    preparing a detailed risk analysis of the GM based bioprocess, and by

     preparing a set of guideline for authorities and end user/site owners.


Milestones and expected results.

Integrated biosensor strains will be constructed facilitating in situ monitoring of PCB remediation. In particular, these tools will allow efficacy monitoring of bioremediation inoculants. The findings will lead to a better understanding of those measures to be applied for optimisation of phytobioremediation technology.

Novel molecular tools will be developed for the identification and unambiguous quantification of genetically modified (GM) bacterial strains in the environment. A detailed impact assessment on the use of the GM strains will underpin the preparation of guidelines on the safe use of this GM technology.
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Last updated: August 22, 2002.